Detection of Babesia ovata from Cattle Reared in Japan and

PCR Detection of Babesia ovata from Cattle Reared in Japan and 1 Clinical Significance of Co-Infection with Theileria orientalis 2 3 Running title: Co-infection of B. ovata and T. orientalis in cattle 4 5 Thillaiampalam Sivakumar, Michihito Tagawa, Takeshi Yoshinari, Adrian P. Ybañez, 6 Ikuo Igarashi, Yuzuru Ikehara, Hiroshi Hata, Seiji Kondo, Kotaro Matsumoto, 7 Hisashi Inokuma, and Naoaki Yokoyama* 8 9 National Research Center for Protozoan Diseases, Obihiro University of Agriculture 10 and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan 11 Department of Clinical Veterinary Science, Obihiro University of Agriculture and 12 Veterinary Medicine, Inada-cho, Obihiro, Hokkaido, Japan 13 Research Center for Glycoscience, National Institute of Advanced Industrial Science 14 and Technology, Tsukuba, Ibaraki, Japan 15 Field Science Center for Northern Biosphere, Hokkaido University, Hidaka-gun, 16 Hokkaido, Japan 17 18 *Corresponding author. Mailing address: National Research Center for Protozoan 19 Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, 20 Obihiro, Hokkaido 080-8555, Japan. Phone: +81-155-49-5649; Fax: +81-155-49-5643; 21 E-mail: [email protected] 22 Copyright © 2012, American Society for Microbiology. All Rights Reserved. J. Clin. Microbiol. doi:10.1128/JCM.00220-12 JCM Accepts, published online ahead of print on 21 March 2012